TRC-00056 | Alpha Crosslaps (αCTx) (Human) ELISA Kit | 96 Well
Product Overview
A Competitive ELISA kit for in vitro quantitative determination of α-CrossLaps concentrations in serum, plasma and other biological fluids.
CrossLaps (CTx) peptide [Glu-Lys-Ala-His-Asp-Gly-Gly-Arg], a part of the C-telopeptide of the alpha 1-chain of type I collagen of bone, is developed as a biochemical marker of bone turnover. The Alpha CrossLaps is intended for use as an indicator of degradation of non-isomerized bone collagen and may be used as an aid in the identification of skeletal metastases in patients with breast and prostate cancer. 96Trace’s Alpha Crosslaps (αCTx) (Human) ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with αCTx. During the reaction, αCTx in the samples or standard competes with αCTx coated on the plate for binding to the anti- αCTx antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of αCTx. The concentration of αCTx in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 93.75-6000 pg/mL, Specificity: No Significant cross-reactivity or interference between Human α-CTx and analogues was observed., Sensitivity: 56.25 pg/mL, Precision:Coefficient of variation is < 10% |
| Sensitivity | 56.25 pg/mL, Precision:Coefficient of variation is < 10% |
Applications
A Competitive ELISA kit for in vitro quantitative determination of α-CrossLaps concentrations in serum, plasma and other biological fluids.
Features & Benefits
Detection Range: 93.75-6000 pg/mL, Specificity: No Significant cross-reactivity or interference between Human α-CTx and analogues was observed., Sensitivity: 56.25 pg/mL, Precision:Coefficient of variation is < 10%.
Storage & Shipping
| Storage | An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4797-100 |
| Supplier CAT | TRC-00056 |
| Analyte / Target | Alpha Crosslaps (αCTx) |
| Category | Cancer Biomarker ELISA Kits |
| Alternate Name | a-CTXI, aCTX-I, A-Cr, Type I Collagen C-Telopeptide-Related Fraction |