5-Lipoxygenase (5-LOX) (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of 5-Lipoxygenase (5-LOX) in Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids.

96Trace's 5-lipoxygenase ELISA kit is based on Sandwich ELISA method. The MicroELISA stripplate provided in this kit has been pre-coated with an antibody specific to 5-LOX. Standards or samples are added to the appropriate MicroELISA stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 5-LOX is added to each MicroELISA stripplate well and after incubation, all unbound components are washed away. The TMB substrate solution is added to each well. The wells that contain 5-LOX and HRP conjugated 5-LOX antibody will appear blue in color and turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 5-LOX. The concentration of 5-LOX in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of 5-Lipoxygenase (5-LOX) in Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of 5-Lipoxygenase (5-LOX).
• Assay format: Sandwich ELISA.
• Sample type: Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids.
• Detection method: Absorbance-450 nm.
• Detection range: 1U/L - 480 U/L, Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%, CV (%) = SD/mean X 100, Sensitivity: 1U/L
• Sensitivity: 1U/L.
• Kit size: 96 Well.

Storage & Shipping

Product Details