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TRC-00011 | 5-Hydroxyindoleacetic Acid (5-HIAA) ELISA Kit | 96 Well

Product Overview

A competitive ELISA kit for the quantitative measurement of 5-HIAA in serum, plasma, culture supernatants and other biological fluids.

5-Hydroxyindoleacetic acid (5-HIAA) is the main metabolite of serotonin. In chemical analysis of urine samples, 5-HIAA is used to determine serotonin levels in the body. Since 5-HIAA is a metabolite of serotonin, testing is most frequently performed for the diagnosis of carcinoid tumors of the enterochromaffin (Kultschitzsky) cells of the small intestine, which release large amounts of serotonin. Values greater than 25 mg per 24 hours (higher if the patient has malabsorption) are strong evidence for carcinoid. 96Trace’s 5-HIAA ELISA kit is a competitive ELISA assay for the quantitative measurement of 5-HIAA in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of 5-HIAA captured from the samples.

Assay Information

Assay FormatCompetitive ELISA
Species ReactivityUniversal
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range1.563 - 100 ng/ml
Sensitivity< 0.938 ng/ml

Applications

This ELISA kit is used for quantitative measurement of 5-HIAA in serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

  • Easy, convenient and time-saving method to measure the level of 5-HIAA in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
  • Detection Range: 1.563 - 100 ng/ml
  • Sensitivity: < 0.938 ng/ml
  • Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
  • This assay has high sensitivity and excellent specificity for detection of 5-HIAA. No significant cross-reactivity or interference between 5-HIAA and analogues was observed.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

96Trace CATE4533-100
Supplier CATTRC-00011
Analyte / Target5-Hydroxyindoleacetic Acid
CategoryNeuroscience ELISA Kits