TRC-00009 | 3-Nitrotyrosine ELISA Kit | 96 Well
Product Overview
An ELISA kit for the quantitative measurement of 3-Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids
3-Nitrotyrosine (3-NT) is a biomarker of nitrogen free radical species modified proteins in systemic autoimmunogenic conditions. This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT. During the reaction 3-NT in the sample or standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 3-NT. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 3-NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Universal |
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatant and other biological fluids. |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Applications
This ELISA kit is used for quantitative measurement of 3-Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Features & Benefits
Easy, convenient and time-saving method to measure the level of 3-Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | K4158-100 |
| Supplier CAT | TRC-00009 |
| Analyte / Target | 3-Nitrotyrosine |
| Category | Oxidative Stress & Cell Damage ELISA Kits |
| Alternate Name | 3-Nitrotyrosine ELISA Kit |