Florfenicol ELISA Kit

Product Overview

A competitive ELISA kit for the quantitative measurement of Florfenicol in Tissue, liver, Egg, Honey, Milk powder, Feed.

Florfenicol is a broad-spectrum, primarily bacteriostatic, antibiotic with a range of activity similar to that of chloramphenicol, including many gram-negative and gram-positive organisms. It has a same mechanism of action as chloramphenicol i.e. inhibition of protein synthesis. Signs of Florfenicol toxicity are varied and include diarrhea and hyperbilirubinemia in horses, diarrhea and decreased feed consumption and rumen activity in cattle. Overdose or prolonged florfenicol administration can cause fatal bone marrow suppression. Florfenicol ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Florfenicol. During the reaction, Florfenicol in the samples or standard competes with Florfenicol coated on the plate for binding to the anti-Florfenicol antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Florfenicol. The concentration of Florfenicol in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Florfenicol in Tissue, liver, Egg, Honey, Milk powder, Feed.

Features & Benefits

• Designed for quantitative measurement of Florfenicol.
• Assay format: Competitive ELISA.
• Sample type: Tissue, liver, Egg, Honey, Milk powder, Feed.
• Detection method: Absorbance (450 nm).
• Detection Range: Tissue, Liver, Honey, Milk - 0.075ppb.
• Sensitivity: 0.15 ppb (ng/mL), Sample recovery rate: Tissue, Liver - 85%±20%.
• Kit size: 96 Well.

Storage & Shipping

Product Details