DYKDDDDK-Tag Protein ELISA Kit

Product Overview

A competitive ELISA kit for the quantitative measurement of DYKDDDDK-Tag Protein in Cell lysate, E. coli lysates.

DYKDDDDK tag has a small unique protein sequence that is commonly fused to N- or C-terminus of a protein for expression. Expression and detection of DYKDDDDK-tag proteins are frequently performed in many academic and industry laboratories because this tag is generally more hydrophilic than other common tags and therefore it is less likely to denature or inactivate proteins to which it is attached. In addition, DYKDDDDK-tag fusion proteins in E. coli or mammalian cell lysate can be easily purified by affinity chromatography using specific resins. Typically DYKDDDDK-tag proteins are detected by SDS-PAGE or western blots but these approaches are generally more laborious, time-consuming and less sensitive. 96Trace’s DYKDDDDK-Tag Protein ELISA Kit is based on the competitive ELISA principle and it is an easy, fast and sensitive method to detect the expressed DYKDDDDK-tag proteins. This detection kit offers ready-to-use reagents, and can detect as low as 100 ng of DYKDDDDK-tag protein in approximately 1.5 hrs.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of DYKDDDDK-Tag Protein in Cell lysate, E. coli lysates.

Features & Benefits

• Designed for quantitative measurement of DYKDDDDK-Tag Protein.
• Assay format: Competitive ELISA.
• Sample type: Cell lysate, E. coli lysates.
• Detection method: Absorbance (650 nm).
• Detection Range: 256 – 10,000 ng/ml, In vitro, quantitative determination of DYKDDDDK-tag fusion proteins, Sensitivity: 100 ng/ml
• Sensitivity: 100 ng/ml.
• Kit size: 96 Well.

Storage & Shipping

Product Details