CHO Host Cell Proteins ELISA kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of CHO Host Cell Proteins in Serum, plasma, tissue homogenates and other biological fluids.

CHO cell line is widely used in the expression of therapeutic protein , which is a cost effective method for production of commercial quantities of a drug substance. One of the goal in designing a downstream process for biopharmaceutical products is to remove any possible contaminants, including host cell proteins. HCPs can still be present at significant concentrations following several purification steps and can even be co-purified and concentrated with the drug substance itself. HCP in the products will result in adverse toxic or immunological reactions which compromise product safety and quality. This it is desirable to reduce the host cell protein contamination to the lowest level. Therefore, during the development of a downstream process, suitable assays must be available to determine the amount of HCP present.. 96Trace’s CHO HCP ELISA kit is a sandwich ELISA assay for the quantitative measurement of CHO HCP in Human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of CHO HCP captured from the samples.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of CHO HCP in serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

• Designed for quantitative measurement of CHO Host Cell Proteins.
• Assay format: Sandwich ELISA.
• Sample type: Serum, plasma, tissue homogenates and other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 0.1 - 100 ng/ml • Sensitivity: < 0.31 ng/ml • Assay Precision: Intra-Assay: CV < 8%.
• Sensitivity: < 0.31 ng/ml • Assay Precision: Intra-Assay: CV < 8%.
• Kit size: 96 Well.

Storage & Shipping

Product Details