IL-24 (Mouse) ELISA Kit
Ref. TRC-00476
Size: 96 Well
Application: Inflammation and immune response biomarker research.
Size: 96 Well
Application: Inflammation and immune response biomarker research.
TRC-00476 | IL-24 (Mouse) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for the quantitative measurement of IL-24 in Plasma,Serum,Other biological fluids.
IL-24 is induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by IL-24 in melanoma cells, but not in normal immortal melanocytes. 96Trace’s IL-24 (Mouse) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-24. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-24 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-24 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse IL-24. The concentration of Mouse IL-24 in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Mouse |
| Sample Type | Plasma,Serum,Other biological fluids |
| Detection Method | Sandwich ELISA, Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 31.25-2000 pg/mL,Sensitivity: 18.75 pg/mL,Precision: Coefficient of variation is < |
| Sensitivity | 18.75 pg/mL,Precision: Coefficient of variation is < |
Features & Benefits
- Designed for quantitative measurement of IL-24.
- Assay format: Sandwich ELISA.
- Sample type: Plasma,Serum,Other biological fluids.
- Detection method: Sandwich ELISA, Absorbance (450 nm).
- Detection Range: 31.25-2000 pg/mL,Sensitivity: 18.75 pg/mL,Precision: Coefficient of variation is <.
- Sensitivity: 18.75 pg/mL,Precision: Coefficient of variation is <.
- Kit size: 96 Well.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| Catalog Number | TRC-00476 |
| Analyte / Target | IL-24 |