IL-1α (Mouse) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of IL-1α in Plasma,Serum,Other biological fluids.

IL-1α is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. 96Trace’s IL-1α (Mouse) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1α. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-1α bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse IL-1 α. The concentration of Mouse IL-1α in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of IL-1α in Plasma,Serum,Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of IL-1α.
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 15.63-1000 pg/mL,Sensitivity: 9.38 pg/mL,Precision: Coefficient of variation is &lt.
• Sensitivity: 9.38 pg/mL,Precision: Coefficient of variation is &lt.
• Kit size: 96 Well.

Storage & Shipping

Product Details