Fibronectin (rat) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of Fibronectin in • Serum & plasma (heparin, EDTA); • Cell culture supernatants.

Fibronectin is a 430 KDa dimeric glycoprotein that exists in 2 forms - cellular and plasma fibronectin. 96Trace’s rat Fibronectin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for rat Fibronectin is coated on a 96-well plate. Standards and test samples are added to the wells and Fibronectin present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Fibronectin is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat Fibronectin captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 15 pg/ml.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Fibronectin in • Serum & plasma (heparin, EDTA); • Cell culture supernatants.

Features & Benefits

• Designed for quantitative measurement of Fibronectin.
• Assay format: Sandwich ELISA.
• Sample type: • Serum & plasma (heparin, EDTA); • Cell culture supernatants.
• Detection method: Absorbance (450 nm).
• Detection Range:156 pg/ml – 10,000 pg/ml. Sensitivity: < 15 pg/ml.
• Sensitivity: < 15 pg/ml.
• Kit size: 96 Well.

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Product Details