Alpha 1-Antitrypsin (Rat) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of Alpha 1-Antitrypsin in Plasma,Serum,Other biological fluids.

Alpha 1-Antitrypsin is an inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin, and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. 96Trace’s Alpha 1-Antitrypsin (Rat) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Alpha 1-Antitrypsin. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Rat Alpha 1-Antitrypsin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of Alpha 1-Antitrypsin bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat Alpha 1-Antitrypsin. The concentration of Rat Alpha 1-Antitrypsinin the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Alpha 1-Antitrypsin in Plasma,Serum,Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of Alpha 1-Antitrypsin.
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Other biological fluids.
• Detection method: Sandwich ELISA, Absorbance (450 nm).
• Detection Range: 6.25-400 ng/mL,Sensitivity: 3.75 ng/mL,Precision: Coefficient of variation is &lt.
• Sensitivity: 3.75 ng/mL,Precision: Coefficient of variation is &lt.
• Kit size: 96 Well.

Storage & Shipping

Product Details