TRC-00638 | Pregnanediol-3-Glucuronide (PDG) ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of PDG in dried fecal, urine and tissue culture samples.
Pregnanediol Glucuronide, C27H44O8, also known as PDG (5β-Pregnan-3a, 20a-diol 3-glucosiduronate) is the major metabolite of progesterone. Progesterone is an essential regulator of human female reproductive function in the uterus, ovary, mammary gland and brain, and plays an important role in non-reproductive tissues such as the cardiovascular system, bone and the central nervous system. 96Trace’s PDG ELISA kit is a competitive ELISA assay for the quantitative measurement of PDG in urine and fecal samples. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A PDG-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to PDG to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound PDG-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | All |
| Sample Type | Cell and tissue culture supernatants,Fecal Extracts,Urine |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.391 - 50 ng/mL |
| Sensitivity | 0.180 ng/mL,Detection Limit: 0.320 ng/mL,Detection Range: 0.391 - 50 ng/mL |
| Detection Limit | 0.320 ng/mL,Detection Range: 0.391 - 50 ng/mL |
Applications
This ELISA kit is used for in vitro quantitative determination of PDG
Features & Benefits
This ELISA kit is used for in vitro quantitative determination of PDG.,Sensitivity: 0.180 ng/mL,Detection Limit: 0.320 ng/mL,Detection Range: 0.391 - 50 ng/mL
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4851-100 |
| Supplier CAT | TRC-00638 |
| Analyte / Target | Pregnanediol-3-Glucuronide |
| Category | Neuroscience ELISA Kits |