TRC-00554 | MOG (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for quantitative measurement of Myelin-oligodendrocyte glycoprotein (MOG) in human serum, plasma, tissue lysates, and other biological fluids
Myelin oligodendrocyte glycoprotein (MOG) is a 26 kDa transmembrane glycoprotein that belongs to the immunoglobulin superfamily. MOG acts either as a cell surface receptor or a cell adhesion molecule. It is expressed exclusively by the oligodendrocytes in the central nervous system (CNS) and on the surface of myelin sheaths. It makes up about 0.05% of the myelin sheath and enables smooth electrical conduction of action potential. The extracellular IgV domain of MOG is accessible to the antibodies present in the extracellular milieu. As a result, the antibodies attack MOG and elicit a demyelinating autoantibody response. MOG antibodies are observed in disorders such as optic neuritis (ON), acute disseminated encephalomyelitis (ADEM), and transverse myelitis (TM). Hence, the detection of MOG antibodies has been implicated in the diagnosis of patients with CNS demyelination disorders. 96Trace’s MOG (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of MOG in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of MOG in the sample or standard.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Tissue lysate Serum Plasma Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.156 – 10 ng/ml |
| Sensitivity | 0.094 ng/ml |
Applications
Sandwich ELISA kit to quantitatively measure MOG in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits
- Detection range: 0.156 – 10 ng/ml
- This Sandwich ELISA is highly sensitive and highly specific for the detection of MOG in human samples. There is no significant cross-reactivity or interference between MOG and analogues
- Sensitivity: 0.094 ng/ml
- Recovery range: 85 - 105% for normal human serum and plasma samples
- Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4963-100 |
| Supplier CAT | TRC-00554 |
| Analyte / Target | MOG |
| Category | Neuroscience ELISA Kits |
| Alternate Name | BTN6, BTNL11, MOGIG2, NRCLP7, MOG |