Troponin I (Rat) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of Troponin I in Plasma, Serum, Other biological fluids.

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. 96Trace's Troponin I ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to rat Troponin I. Standards or samples were added to the micro ELISA plate that reacts with biotinylated detection antibody specific for rat Troponin I and Avidin-Horseradish Peroxidase (HRP) conjugate which on the addition of substrate form blue color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of rat Troponin I. The concentration of rat Troponin I in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Troponin I in Plasma, Serum, Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of Troponin I.
• Assay format: Sandwich ELISA.
• Sample type: Plasma, Serum, Other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 15.63-1000 pg/ml, Precision: Coefficient of variation is < 10%.
• Sensitivity: 9.38 pg/ml, Detection Range: 15.63-1000 pg/ml, Precision: Coefficient of variation is < 10%.
• Kit size: 96 Well.

Storage & Shipping

Product Details