IL-2 (Mouse) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of IL-2 in Plasma,Serum,Other biological fluids.

Interleukin-2 (Il-2) is produced by T-cells in response to antigenic or mitogenic stimulation. It is is required for T-cell proliferation and other activities crucial to regulation of the immune response. IL-2 can also stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. 96Trace’s IL-2 (Mouse) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-2 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse IL-2. The concentration of Mouse IL-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of IL-2 in Plasma,Serum,Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of IL-2.
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Other biological fluids.
• Detection method: Sandwich ELISA, Absorbance (450 nm).
• Detection Range: 15.63-1000 pg/mL,Specificity: This kit recognizes Mouse IL-2 in samples. No significant cross-reactivity or interference between Mouse IL-2 and analogues was observed.,Precision: Coefficient of variation is &lt.
• Sensitivity: 9.38 pg/mL,Detection Range: 15.63-1000 pg/mL,Specificity: This kit recognizes Mouse IL-2 in samples. No significant cross-reactivity or interference between Mouse IL-2 and analogues was observed.,Precision: Coefficient of variation is < 10%.
• Kit size: 96 Well.

Storage & Shipping

Product Details