Estradiol (rat) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of Estradiol in • Serum & plasma (EDTA/Citrate), urine; • Cell culture medium, tissue homogenates and cell lysates.

Estradiol (E2 or 17β-estradiol) is the predominant female reproductive hormone secreted by the ovaries. Smaller amounts of estradiol are also produced by the adrenal cortex and by the testes. 96Trace’s rat Estradiol E2 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Rat Estradiol E2 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Estradiol E2 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat Estradiol E2 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 2 – 50 ng/L.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Estradiol in • Serum & plasma (EDTA/Citrate), urine; • Cell culture medium, tissue homogenates and cell lysates.

Features & Benefits

• Designed for quantitative measurement of Estradiol.
• Assay format: Sandwich ELISA.
• Sample type: • Serum & plasma (EDTA/Citrate), urine; • Cell culture medium, tissue homogenates and cell lysates.
• Detection method: Absorbance (450 nm).
• Detection Range:2 – 50 ng/L.
• Kit size: 96 Well.

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Product Details