Chlamydia Trachomatis IgA ELISA Kit

Product Overview

Chlamydia Trachomatis IgA ELISA KIt is intended for the determination of specific IgA antibody to Chlamydia in a single human serum sample.

Chlamydia Trachomatis is one of the most common human pathogens. Of the 15 recognized serotypes, 4 (A, B, Ba, and C) have been shown to cause hyperendemic blinding trachoma, a disease which afflicts hundreds of millions of people in developing countries. Three serotypes (L-1, L-2, and L-3) are the causes of lymphogranuloma venereum (LGV), a sexually transmitted systemic disease.High levels of anti-Chlamydia IgG antibody are of diagnostic value in chronic or systemic infections such as salpingitis, mechanical infertility, perihepatitis, epididymitis, Reiter’s syndrome and pneumonitis.
96Trace's Chlamydia Trachomatis ELISA Kit employs the LGV type 2 broadly reacting antigen of Chlamydia Trachomatis. It will detect Chlamydia Trachomatis, Chlamydia Psittaci and Chlamydia Pneumoniae (TWAR) antibodies. Purified Chlamydia Trachomatis antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the Chlamydia Trachomatis IgA specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgA specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.

Assay Information

Applications

3110

Features & Benefits

Accuracy (Overall agreement) = (A+D) / (A+B+C+D) = 38 / 40 = 95%, Sensitivity = A / (A+B)= 5 / (5 + 0) = 100%, Specificity = D / (C + D) = 33 / (33+2) = 94%

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