TRC-00521 | Lipoxin A4 ELISA Kit | 96 Well
Product Overview
A Competitive ELISA kit for in vitro quantitative determination of Lipoxin A4 concentrations in serum, plasma and other biological fluids.
Lipoxin A4 (LXA4), an endogenous lipoxygenase-derived eicosanoid mediator, has potent dual pro-resolving and anti-inflammatory properties. Endogenous transcellular biosynthesis of LXA4 occurs via interaction of leukocytes with epithelium, endothelium or platelets. Lipoxins (LXs) or the lipoxygenase interaction products are generated from arachidonic acid via sequential actions of lipoxygenases and subsequent reactions to give specific trihydroxytetraene-containing eicosanoids. 96Trace Lipoxin A4 ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Lipoxin A4. During the reaction, Lipoxin A4 in the samples or standard competes with Lipoxin A4 coated on the plate for binding to the anti-Lipoxin A4 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Lipoxin A4. The concentration of Lipoxin A4 in the samples can be calculated by comparing the OD of the samples to the standard curve
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Universal |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.78-50 ng/mL |
| Sensitivity | 0.47 ng/mL, Precision: Coefficient of variation is < 10%., Detection Range: 0.78-50 ng/mL |
Applications
in vitro quantitative measurement of Lipoxin A4 in rat serum, plasma and other biological samples.
Features & Benefits
Specificity: This kit recognizes LXA4 in samples. No Significant cross-reactivity or interference between LXA4 and analogues was observed., Sensitivity: 0.47 ng/mL, Precision: Coefficient of variation is < 10%., Detection Range: 0.78-50 ng/mL
Storage & Shipping
| Storage | An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4788-100 |
| Supplier CAT | TRC-00521 |
| Analyte / Target | Lipoxin A4 |
| Category | Immunology & Inflammation ELISA Kits |
| Alternate Name | 3110 |