TRC-00038 | AIRE (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for quantitative measurement of Autoimmune regulator (AIRE) in human serum, plasma, tissue lysates, and other biological fluids
Autoimmune regulator (AIRE) is a key transcription factor that is expressed in the epithelial cells of the thymic medulla (mTECs). Besides the thymus, AIRE is also expressed in peripheral lymphoid organs and tissues such as the spleen, lymph nodes, embryonic liver, testis, and ovaries. However, its role in peripheral tolerance is less known. AIRE regulates immune tolerance by clearing auto-reactive T cells and induce the production of Treg cells. AIRE mutations result in a rare autoimmune disorder called autoimmune polyglandular syndrome type 1 (APS-1), also known as autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). 96Trace’s AIRE (Human) ELISA kit is to quantitatively measure the amount of AIRE in human serum, plasma, and other biological fluids. The kit is based on the Sandwich ELISA principle. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of AIRE in the sample or standard.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Other biological fluids Serum Tissue lysate Plasma |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.156 – 10 ng/ml |
| Sensitivity | 0.094 ng/ml |
Applications
Sandwich ELISA kit to quantitatively measure AIRE in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits
- Recovery range: 85 - 105% for normal human serum and plasma samples
- Detection range: 0.156 – 10 ng/ml
- This Sandwich ELISA is highly sensitive and highly specific for the detection of AIRE in human samples. There is no significant cross-reactivity or interference between AIRE and analogues
- Sensitivity: 0.094 ng/ml
- Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4971-100 |
| Supplier CAT | TRC-00038 |
| Analyte / Target | AIRE |
| Category | Immunology & Inflammation ELISA Kits |
| Alternate Name | APS1, APSI, PGA1, AIRE1, APECED, AIRE, APECED Protein |