TIMP-3 (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of TIMP-3 in Plasma,Serum,Other biological fluids.

TIMP-3 belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. 96Trace’s TIMP-3 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-3. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of TIMP-3 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human TIMP-3. The concentration of Human TIMP-3 in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of TIMP-3 in Plasma,Serum,Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of TIMP-3.
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Other biological fluids.
• Detection method: Sandwich ELISA, Absorbance (450 nm).
• Detection Range: 62.50-4000 pg/mL,Sensitivity: 37.50 pg/mL,Precision: Coefficient of variation is &lt.
• Sensitivity: 37.50 pg/mL,Precision: Coefficient of variation is &lt.
• Kit size: 96 Well.

Storage & Shipping

Product Details