Surfactant Protein-D (SP-D) (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of Surfactant Protein-D (SP-D) in Plasma,Serum,Tissue lysate,Other biological fluids.

Surfactant protein D (SP-D) is a type of lectin that belongs to the family of collectins, which are a group of collagen-containing C-type lectins. It is produced and secreted by type II alveolar cells, bronchiolar epithelial cells, and Clara cells. SP-D acts as a pattern recognition receptor; it binds to several pathogens of bacterial, fungal, and viral origin and promotes their clearance via phagocytosis. Additionally, SP-D is known to aggregate allergens and assist in their removal by stimulating an allergenic response. SP-D also binds to inflammatory ligands and inhibits pulmonary inflammation. Due to its role in phagocytosis and anti-inflammation, SP-D could serve as an efficient target for the treatment of pulmonary diseases such as chronic obstructive pulmonary disease, asthma, acute interstitial pneumonia (ARDS), and bronchopulmonary dysplasia. 96Trace’s SP-D (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the concentration of SP-D in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of SP-D in the sample or standard.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Surfactant Protein-D (SP-D) in Plasma,Serum,Tissue lysate,Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of Surfactant Protein-D (SP-D).
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Tissue lysate,Other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 1.563 - 100 ng/ml.
• Sensitivity: 0.938 ng/ml,Detection range: 1.563 - 100 ng/ml.
• Kit size: 96 Well.

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Product Details