Proinsulin (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of Proinsulin in Human serum, plasma, tissue homogenates and other biological fluids.

Proinsulin is synthesized in the beta cells of the pancreas and is the precursor molecule for insulin. Most proinsulin is converted to insulin and C-Peptide, which are secreted in equimolar amounts into the blood. About 15% is not converted and is released as proinsulin. The biological activity of proinsulin is only about 10% of Insulin, but the half-life of proinsulin is three times as long as insulin. The level of proinsulin in serum can be a reflection of ß cell status. Both IDDM and NIDDM are characterized by dysfunction of the pancreatic beta cells. Elevated proinsulin levels have been noted at the onset of IDDM and in healthy siblings of IDDM patients. Proinsulin levels may also be increased in patients with established NIDDM. 96Trace’s Proinsulin ELISA kit is a sandwich ELISA assay for measuring the quantity of human Proinsulin in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Proinsulin in Human serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

• Designed for quantitative measurement of Proinsulin.
• Assay format: Sandwich ELISA.
• Sample type: Human serum, plasma, tissue homogenates and other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 15.6 – 1000 pg/ml.
• Assay Time: 90 minutes.
• Kit size: 96 Well.

Storage & Shipping

Product Details