Presepsin (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the qualitative detection of Presepsin in Plasma,Serum,Other biological fluids,Tissue and cell lysates.

Presepsin is a soluble subtype CD14. It is considered a novel biomarker for sepsis diagnostic and prognosis evaluation. 96Trace’s Presepsin ELISA kit is a sandwich ELISA assay for the quantitative measurement of Presepsin in human serum, plasma and cell culture supernatants. Anti-Presepsinantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Presepsin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Presepsin amount of sample captured in plate.

Assay Information

Performance Data

Applications

This ELISA kit is used for qualitative detection of Presepsin in Plasma,Serum,Other biological fluids,Tissue and cell lysates.

Features & Benefits

• Designed for qualitative detection of Presepsin.
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Other biological fluids,Tissue and cell lysates.
• Detection method: Colormetric.
• Detection Range: 0.156-10 ng/ml.
• Sensitivity: < 0.094 ng/ml,Assay Precision: Intra-Assay: CV < 8%.
• Kit size: 96 Well.

Storage & Shipping

Product Details