Neprilysin (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of Neprilysin in Plasma,Serum,Tissue lysate,Other biological fluids.

Neprilysin (MME, CD10) is a zinc-dependent membrane metallopeptidase located on the surface of neutrophils, endothelial cells, and a variety of tissues such as brain, heart, kidneys, and lungs. It catalyzes the degradation of peptides such as atrial (ANP), B-type (BNP), and C-type (CNP) natriuretic peptides, Angiotensin-II, Bradykinin, and Endothelin-1. It also degrades amyloid-beta peptide in the brain and slows the progression of Alzheimer’s disease. Additionally, it is an important cell surface marker in the diagnosis of hematologic diseases such as human acute lymphocytic leukemia (ALL), Burkitt lymphoma, and diffuse large B-cell lymphoma. 96Trace’s Neprilysin (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of Neprilysin in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed away with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Neprilysin in the sample or standard.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Neprilysin in Plasma,Serum,Tissue lysate,Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of Neprilysin.
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Tissue lysate,Other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 0.156 - 10 ng/ml.
• Sensitivity: 0.094 ng/ml,Detection range: 0.156 - 10 ng/ml.
• Kit size: 96 Well.

Storage & Shipping

Product Details