IL-15 (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of IL-15 in Plasma,Serum,Other biological fluids.

IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of the IL2 receptor, including IL-2RB and probably IL-2RG but not IL-2RA. IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection. 96Trace’s IL-15 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-15. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IL-15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-15 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-15. The concentration of Human IL-15 in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of IL-15 in Plasma,Serum,Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of IL-15.
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Other biological fluids.
• Detection method: Sandwich ELISA, Absorbance (450 nm).
• Detection Range: 15.63-1000 pg/mL.
• Sensitivity: 9.38 pg/mL,Precision: Coefficient of variation is &lt.
• Kit size: 96 Well.

Storage & Shipping

Product Details