IGFBP-3 (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of IGFBP-3 in Plasma,Serum,Tissue lysate,Other biological fluids.

Insulin-like growth factor binding protein-3 (IGFBP-3) is a 264 amino acid peptide that belongs to the family of IGF-binding proteins. It is expressed in multiple tissues. One of the main functions of IGFBP-3 is that it regulates the transport, bioavailability, and half-life of insulin-like growth factors, especially IGF-1. IGFBP-3 is inversely correlated with cancer. Increased levels of IGFBP-3 in the serum inhibit proliferation and promote apoptosis of cancer cells, thus serving as a potential target for cancer research. 96Trace’s IGFBP-3 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of IGFBP-3 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of IGFBP-3 in the sample or standard.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of IGFBP-3 in Plasma,Serum,Tissue lysate,Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of IGFBP-3.
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Tissue lysate,Other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 0.391 - 25 ng/ml.
• Sensitivity: 0.234 ng/ml,Assay Precision: Intra-Assay CV &lt.
• Kit size: 96 Well.

Storage & Shipping

Product Details