Hydroxylysine (Human) ELISA Kit
Ref. TRC-00436
Size: 96 Well
Application: Bone, cartilage and extracellular matrix research.
Size: 96 Well
Application: Bone, cartilage and extracellular matrix research.
TRC-00436 | Hydroxylysine (Human) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for the quantitative measurement of Hydroxylysine in Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids, Tissue and cell lysates.
Hydroxylysine (Hyl) is an amino acid which arises from a post-translational hydroxy modification of lysine. It is most widely known as a component of collagen. Hydroxylysine (Human) ELISA Kit uses a double-antibody sandwich enzyme-linked immunosorbent one-step process assay to assay the level of hydroxylysine (Hyl) in samples.
Standard, test sample and HRP-labeled hydroxylysine (Hyl) antibodies were added to enzyme wells which are Pre-coated with hydroxylysine (Hyl) antibody, then carry out incubation and wash to remove the uncombined enzyme. Upon adding Chromogen Solution A and B, the color of the liquid will change into blue, and the reaction with the acid will cause the color to become yellow. The depth of color and the concentration of the hydroxylysine(Hyl) sample are positively correlated.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids, Tissue and cell lysates |
| Detection Method | Absorbance-450 nm |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 9.3ng/ml- 300ng/ml, Accuracy: Standard linear regression correlation coefficient R with the expected value of the concentration, greater than or equal to 0.9900 |
Applications
This ELISA kit is used for quantitative measurement of Hydroxylysine in Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids, Tissue and cell lysates.
Storage & Shipping
| Storage | +4°C |
| Shipping | Room Temperature |
| Shelf Life | 12 Months |
Product Details
| Catalog Number | TRC-00436 |
| Analyte / Target | Hydroxylysine |
| Alternate Name | hydroxylysine, Hyl |