GDF-15 (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of GDF-15 in Plasma,Serum,Tissue lysate,Other biological fluids.

Growth Differentiation Factor 15 (GDF-15), also known as Macrophage Inhibitory Cytokine-1 (MIC-1), belongs to the superfamily of transforming growth factor-beta (TGF-β). During normal conditions, GDF-15 is expressed at low levels in organs such as kidney, stomach, bladder, and placenta. Under stress conditions, the expression is upregulated by various stress-related proteins such as IL-1β, TNF-α, IL-2 in a tissue-specific manner. Elevated expressions are observed in diseased states such as cancer, inflammation, cardiovascular disease, and kidney disease. Hence, GDF-15 serves as an efficient biomarker for cancer and cardiovascular disease. 96Trace’s GDF-15 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of GDF-15 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added, and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of GDF-15 in the sample or standard.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of GDF-15 in Plasma,Serum,Tissue lysate,Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of GDF-15.
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Tissue lysate,Other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 23.438 - 1500 pg/ml.
• Sensitivity: 14.063 pg/ml,Recovery range: 85 - 105% for normal human serum and plasma samples,This Sandwich ELISA is highly sensitive and highly specific for the detection of GDF-15 in human samples. There is no significant cross-reactivity or interference of GDF-15 with analogues,Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%,Detection range: 23.438 - 1500 pg/ml
• Kit size: 96 Well.

Storage & Shipping

Product Details