Filaggrin (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of Filaggrin in Other biological fluids; Tissue lysate; Plasma; Serum.

Filaggrin (FLG) is a critical structural protein in the stratum corneum of the skin epidermis. The protein is synthesized as a precursor (Profilaggrin) and is localized in keratohyalin granules in the stratum granulosum. During terminal differentiation of epidermal cells, Profilaggrin undergoes proteolysis to yield monomeric filaggrin units. Filaggrin regulates epidermal homeostasis. It also maintains skin barrier function. Additionally, it promotes the aggregation of keratin intermediate filaments to form a keratin network which contributes to the physical strength of the skin. Various proteases hydrolyze Filaggrin to yield amino acids and amino acid derivatives that function as natural moisturizing factors in the stratum corneum. Mutations in Filaggrin result in skin disorders such as atopic dermatitis and eczema. Autoantibodies in rheumatoid arthritis (RA) recognize citrullinated residues in Filaggrin. Hence antibodies to Filaggrin are used as markers to detect RA. 96Trace’s Filaggrin (Human) ELISA kit is used to detect Filaggrin in human serum, plasma, and other biological fluids. The kit is based on the Sandwich principle. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of Filaggrin in the sample or standard.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Filaggrin in Other biological fluids; Tissue lysate; Plasma; Serum.

Features & Benefits

• Designed for quantitative measurement of Filaggrin.
• Assay format: Sandwich ELISA.
• Sample type: Other biological fluids; Tissue lysate; Plasma; Serum.
• Detection method: Absorbance (450 nm).
• Detection range: 0.313 – 20 ng/ml
• Sensitivity: 0.188 ng/ml
• Kit size: 96 Well.

Storage & Shipping

Product Details