BRCA1 (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of BRCA1 in Human serum, plasma, tissue homogenates and other biological fluids.

Breast cancer type 1 susceptibility protein (BRCA1) is an E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. 96Trace’s BRCA1 ELISA kit is a sandwich ELISA assay for the quantitative measurement of human BRCA1 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human BRCA1 captured from the samples.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of BRCA1 in human serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

• Designed for quantitative measurement of BRCA1.
• Assay format: Sandwich ELISA.
• Sample type: Human serum, plasma, tissue homogenates and other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 0.312 – 20 ng/ml
• Sensitivity: < 0.188 ng/ml
• Kit size: 96 Well.

Storage & Shipping

Product Details