Anti-Filgrastim (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of Anti-Filgrastim in Human serum and plasma.

Filgrastim is a recombinant, non-pegylated human granulocyte colony-stimulating factor (G-CSF) analog. It binds to the G-CSF receptor and stimulates the production of neutrophils in the bone marrow. As a G-CSF analog, it controls the proliferation of committed progenitor cells and influences their maturation into mature neutrophils. Filgrastim is used in patients with acute myeloid leukemia receiving induction or consolidation chemotherapy. It is also used in cancer patients receiving bone marrow transplants. However, some patients develop unwanted immunogenicity, which leads to the production of anti-drug antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects. 96Trace’s BioSimTM anti-Filgrastim (Human) ELISA kit is designed to detect antibodies against Filgrastim with high specificity and sensitivity in biological matrices. The kit is based on the sandwich principle. Controls and samples are incubated in the microtiter plate coated with the drug Filgrastim. After incubation, the wells are washed, and the enzymatic activity is detected by the addition of chromogen-substrate. The enzyme-substrate reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of antibodies specific for Filgrastim present in the samples and standards. The quantitative test results can be determined using the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of antibody against Filgrastim in human serum and plasma

Features & Benefits

• Designed for quantitative measurement of Anti-Filgrastim.
• Assay format: Sandwich ELISA.
• Sample type: Human serum and plasma.
• Detection method: Absorbance (450 nm).
• Detection Range: 3.1 - 50 ng/ml
• Sensitivity: 3.1 ng/ml
• Kit size: 96 Well.

Storage & Shipping

Product Details