TRC-00348 | Filaggrin (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA Kit for quantitative measurement of Filaggrin in human serum, plasma, tissue lysates, and other biological fluids
Filaggrin (FLG) is a critical structural protein in the stratum corneum of the skin epidermis. The protein is synthesized as a precursor (Profilaggrin) and is localized in keratohyalin granules in the stratum granulosum. During terminal differentiation of epidermal cells, Profilaggrin undergoes proteolysis to yield monomeric filaggrin units. Filaggrin regulates epidermal homeostasis. It also maintains skin barrier function. Additionally, it promotes the aggregation of keratin intermediate filaments to form a keratin network which contributes to the physical strength of the skin. Various proteases hydrolyze Filaggrin to yield amino acids and amino acid derivatives that function as natural moisturizing factors in the stratum corneum. Mutations in Filaggrin result in skin disorders such as atopic dermatitis and eczema. Autoantibodies in rheumatoid arthritis (RA) recognize citrullinated residues in Filaggrin. Hence antibodies to Filaggrin are used as markers to detect RA. 96Trace’s Filaggrin (Human) ELISA kit is used to detect Filaggrin in human serum, plasma, and other biological fluids. The kit is based on the Sandwich principle. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of Filaggrin in the sample or standard.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Other biological fluids Tissue lysate Plasma Serum |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.313 – 20 ng/ml |
| Sensitivity | 0.188 ng/ml |
Applications
Sandwich ELISA kit to quantitatively measure FLG in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits
- This Sandwich ELISA is highly sensitive and highly specific for the detection of FLG in human samples. There is no significant cross-reactivity or interference between FLG and analogues
- Recovery range: 85 - 105% for normal human serum and plasma samples
- Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
- Detection range: 0.313 – 20 ng/ml
- Sensitivity: 0.188 ng/ml
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4970-100 |
| Supplier CAT | TRC-00348 |
| Analyte / Target | Filaggrin |
| Category | Epigenetics & DNA Damage ELISA Kits |
| Alternate Name | ATOD2, FLG, Filaggrin, Epidermal Filaggrin |