TRC-00630 | PGFM ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of PGFM in fecal, urine, serum, plasma and tissue culture samples.
In many species, uterine and placental Prostaglandin F2alpha (PGF2a) is involved in the regulation of reproductive and pregnancy-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. PGFM has been suggested as a useful non-invasive marker of pregnancy when measured in both urine and fecal samples. 96Trace’s PGFM ELISA kit is a competitive ELISA assay for the quantitative measurement of PGFM in fecal extracts, urine, serum and plasma samples. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to PGFM to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound PGFM -peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | All |
| Sample Type | Fecal Extracts,Plasma,Cell and tissue culture supernatants,Serum,Urine |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 50- 3200 pg/mL,This ELISA kit is used for in vitro quantitative determination of PGFM |
| Sensitivity | 20.8 pg/mL,Detection Range: 50- 3200 pg/mL,This ELISA kit is used for in vitro quantitative determination of PGFM |
| Detection Limit | 46.2 pg/mL,Sensitivity:20.8 pg/mL,Detection Range: 50- 3200 pg/mL,This ELISA kit is used for in vitro quantitative determination of PGFM |
Applications
This ELISA kit is used for in vitro quantitative determination of PGFM
Features & Benefits
Detection Limit: 46.2 pg/mL,Sensitivity:20.8 pg/mL,Detection Range: 50- 3200 pg/mL,This ELISA kit is used for in vitro quantitative determination of PGFM
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4853-100 |
| Supplier CAT | TRC-00630 |
| Analyte / Target | PGFM |
| Category | Cytokine & Growth Factor ELISA Kits |