IL-1α (Mouse) ELISA Kit

Product Overview

A Sandwich ELISA kit for the quantitative measurement of IL-1α in mouse serum, plasma and other biological fluids.

IL-1α is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. 96Trace’s IL-1α (Mouse) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1α. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-1α bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse IL-1 α. The concentration of Mouse IL-1α in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

in vitro quantitative measurement of IL-1α in mouse serum, plasma and other biological fluids.

Features & Benefits

Specificity: No Significant cross-reactivity or interference between Mouse IL-1α and analogues was observed.,Detection Range: 15.63-1000 pg/mL,Sensitivity: 9.38 pg/mL,Precision: Coefficient of variation is < 10%

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Product Details