TRC-00468 | IL-1α (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for the quantitative measurement of IL-1α in human serum, plasma and other biological fluids.
IL-1α is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. 96Trace’s IL-1α (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1α. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-1α bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-1 α. The concentration of Human IL-1α in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 3.91-250 pg/mL |
| Sensitivity | 2.35 pg/mL, Precision: Coefficient of variation is < 10%, Specificity: No Significant cross-reactivity or interference between Human IL-1α and analogues was observed., Detection Range: 3.91-250 pg/mL |
Applications
in vitro quantitative measurement of IL-1α in human serum, plasma and other biological fluids.
Features & Benefits
Sensitivity: 2.35 pg/mL, Precision: Coefficient of variation is < 10%, Specificity: No Significant cross-reactivity or interference between Human IL-1α and analogues was observed., Detection Range: 3.91-250 pg/mL
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4802-100 |
| Supplier CAT | TRC-00468 |
| Analyte / Target | IL-1α |
| Category | Cytokine & Growth Factor ELISA Kits |
| Alternate Name | IL1A, IL1-A, IL1, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha |