IFN-gamma (Human) ELISA Kit

Product Overview

A Sandwich ELISA kit for the quantitative measurement of human IFN-γ in plasma, tissue homogenates and other biological fluids.

IFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. 96Trace’s IFN-gamma (Human) ELISA kit is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-gamma. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IFN-gamma and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IFN-gamma bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IFN-gamma. The concentration of Human IFN-gamma in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

in vitro quantitative measurement of human IFN-γ in plasma, tissue homogenates and other biological fluids.

Features & Benefits

Specificity: No Significant cross-reactivity or interference between Human IFN-γ and analogues was observed.,Detection Range: 15.63-1000 pg/mL,Precision: Coefficient of variation is < 10%.,Sensitivity: 9.38 pg/mL

Storage & Shipping

Product Details