TRC-00322 | Estradiol (human) ELISA Kit | 96 Well
Product Overview
Sensitive, Colorimetric Assay
Estradiol (E2 or 17β-estradiol) is the predominant female reproductive hormone secreted by the ovaries. Smaller amounts of estradiol are also produced by the adrenal cortex, and by the testes in men. 96Trace’s human Estradiol E2 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human Estradiol E2 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Estradiol E2 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Estradiol E2 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 2 – 50 pmol/L.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | human |
| Sample Type | • Serum & plasma (EDTA/Citrate), urine • Cell culture medium, tissue homogenates and cell lysates |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 2 – 50 pmol/L |
Applications
This ELISA kit is used for quantitative detection of Estradiol, establishing normal range.
Features & Benefits
- Easy, convenient and time-saving method to assay for human Estradiol E2.
- Detection Range:2 – 50 pmol/L.
- This ELISA kit shows no species cross-reactivity.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | K3829-100 |
| Supplier CAT | TRC-00322 |
| Analyte / Target | Estradiol |
| Category | Cytokine & Growth Factor ELISA Kits |
| Alternate Name | Estradiol (human) ELISA kit |