TRC-00314 | Epiandrosterone ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of Epiandrosterone in Saliva, Urine, Extracted Serum or Plasma, Fecal Extracts samples
Epiandrosterone, or 3ß-androsterone, also known as 3ß-hydroxy-5a-androstan-17-one or 5α-androstan-3ß-ol17-one, is a steroid hormone with weak androgenic activity. It is a natural metabolite of dehydroepiandrosterone (DHEA) via the 5α-reductase enzyme. 96Trace’s Epiandrosterone ELISA kit is a competitive ELISA assay for the quantitative measurement of Epiandrosterone in urine, saliva, and fecal samples, or in extracted serum and plasma. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An Epiandrosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to Epiandrosterone to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound Epiandrosterone -peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | All |
| Sample Type | Extracted Serum or Plasma,Cell and tissue culture supernatants,Dried Fecal Extracts,Urine,Saliva |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.098 - 100 ng/mL |
| Sensitivity | 0.120 ng/mL.,This ELISA kit is used for in vitro quantitative determination of Epiandrosterone.,Detection Range: 0.098 - 100 ng/mL |
| Detection Limit | 0.107 ng/mL,Sensitivity:0.120 ng/mL.,This ELISA kit is used for in vitro quantitative determination of Epiandrosterone.,Detection Range: 0.098 - 100 ng/mL |
Applications
This ELISA kit is used for in vitro quantitative determination of Epiandrosterone
Features & Benefits
Detection Limit: 0.107 ng/mL,Sensitivity:0.120 ng/mL.,This ELISA kit is used for in vitro quantitative determination of Epiandrosterone.,Detection Range: 0.098 - 100 ng/mL
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4852-100 |
| Supplier CAT | TRC-00314 |
| Analyte / Target | Epiandrosterone |
| Category | Cytokine & Growth Factor ELISA Kits |