TRC-00823 | Troponin I (Rat) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for in vitro quantitative determination of rat Troponin I concentrations in serum, plasma and other biological fluids.
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. 96Trace's Troponin I ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to rat Troponin I. Standards or samples were added to the micro ELISA plate that reacts with biotinylated detection antibody specific for rat Troponin I and Avidin-Horseradish Peroxidase (HRP) conjugate which on the addition of substrate form blue color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of rat Troponin I. The concentration of rat Troponin I in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Rat |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15.63-1000 pg/ml, Precision: Coefficient of variation is < 10% |
| Sensitivity | 9.38 pg/ml, Detection Range: 15.63-1000 pg/ml, Precision: Coefficient of variation is < 10% |
Applications
3110
Features & Benefits
Specificity: No Significant cross-reactivity or interference between Rat Tn-I and analogues was observed., Sensitivity: 9.38 pg/ml, Detection Range: 15.63-1000 pg/ml, Precision: Coefficient of variation is < 10%.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4737-100 |
| Supplier CAT | TRC-00823 |
| Analyte / Target | Troponin I |
| Category | Cardiovascular ELISA Kits |
| Alternate Name | TNNI, TNI, Tn-I |