TRC-00821 | Troponin I (Human) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for in vitro quantitative determination of human Troponin I concentrations in serum, plasma and other biological fluids.
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. 96Trace's Troponin I ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to human Troponin I. Standards or samples were added to the micro ELISA plate that reacts with biotinylated detection antibody specific for human Troponin I and Avidin-Horseradish Peroxidase (HRP) conjugate which on the addition of substrate form blue color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of human Troponin I. The concentration of human Troponin I in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.16-10 ng/ml |
| Sensitivity | 0.10 ng/ml, Detection Range: 0.16-10 ng/ml |
Applications
3110
Features & Benefits
Specificity: No Significant cross-reactivity or interference between Human Tn-I and analogues was observed., Coefficient of variation is < 10%, Sensitivity: 0.10 ng/ml, Detection Range: 0.16-10 ng/ml
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4735-100 |
| Supplier CAT | TRC-00821 |
| Analyte / Target | Troponin I |
| Category | Cardiovascular ELISA Kits |
| Alternate Name | Tn-I |