TRC-00055 | Alpha 1-Antitrypsin (Rat) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for the quantitative measurement of rat Alpha 1-Antitrypsin in serum, plasma and other biological fluids.
Alpha 1-Antitrypsin is an inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin, and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. 96Trace’s Alpha 1-Antitrypsin (Rat) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Alpha 1-Antitrypsin. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Rat Alpha 1-Antitrypsin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of Alpha 1-Antitrypsin bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat Alpha 1-Antitrypsin. The concentration of Rat Alpha 1-Antitrypsinin the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Rat |
| Sample Type | Plasma,Serum,Other biological fluids |
| Detection Method | Sandwich ELISA, Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 6.25-400 ng/mL,Sensitivity: 3.75 ng/mL,Precision: Coefficient of variation is < |
| Sensitivity | 3.75 ng/mL,Precision: Coefficient of variation is < |
Applications
In vitro quantitative determination of rat Alpha 1-Antitrypsin concentrations in serum, plasma and other biological fluids
Features & Benefits
Detection Range: 6.25-400 ng/mL,Sensitivity: 3.75 ng/mL,Precision: Coefficient of variation is < 10%,Specificity: No Significant cross-reactivity or interference between Rat α1-AT and analogues was observed.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4823-100 |
| Supplier CAT | TRC-00055 |
| Analyte / Target | Alpha 1-Antitrypsin |
| Category | Cardiovascular ELISA Kits |
| Alternate Name | SERPINA1, A1A, A1AT, AAT, PI, PI1, PRO2275 |