6-Keto-PGF1 α ELISA Kit
Ref. TRC-00013
Size: 96 Well
Application: Cardiovascular and vascular biomarker research.
Size: 96 Well
Application: Cardiovascular and vascular biomarker research.
TRC-00013 | 6-Keto-PGF1 α ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of 6-Keto-PGF1 α in Plasma, Serum, Other biological fluids.
6-keto-PGF1α is a stable hydrolysis product of prostacyclin. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia. 96Trace 6-keto-PGF1α ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with 6-keto-PGF1α. During the reaction, 6-keto-PGF1αin the samples or standard competes with 6-keto-PGF1α coated on the plate for binding to the anti-6-keto-PGF1αantibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of 6-keto-PGF1α. The concentration of 6-keto-PGF1αin the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Universal |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15.63-1000 pg/mL, Specificity: This kit recognizes 6-keto-PGF1a in samples. No Significant cross-reactivity or interference between 6-keto-PGF1a and analogues was observed |
| Sensitivity | 9.38 pg/mL, Precision: Coefficient of variation is < 10%., Detection Range: 15.63-1000 pg/mL, Specificity: This kit recognizes 6-keto-PGF1a in samples. No Significant cross-reactivity or interference between 6-keto-PGF1a and analogues was observed |
Applications
This ELISA kit is used for quantitative measurement of 6-Keto-PGF1 α in Plasma, Serum, Other biological fluids.
Features & Benefits
- Designed for quantitative measurement of 6-Keto-PGF1 α.
- Assay format: Competitive ELISA.
- Sample type: Plasma, Serum, Other biological fluids.
- Detection method: Absorbance (450 nm).
- Detection Range: 15.63-1000 pg/mL, Specificity: This kit recognizes 6-keto-PGF1a in samples. No Significant cross-reactivity or interference between 6-keto-PGF1a and analogues was observed.
- Sensitivity: 9.38 pg/mL, Precision: Coefficient of variation is < 10%., Detection Range: 15.63-1000 pg/mL, Specificity: This kit recognizes 6-keto-PGF1a in samples. No Significant cross-reactivity or interference between 6-keto-PGF1a and analogues was observed.
- Kit size: 96 Well.
Storage & Shipping
| Storage | An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| Catalog Number | TRC-00013 |
| Analyte / Target | 6-Keto-PGF1 α |
| Alternate Name | 3110 |