TRC-00800 | TNF-α (Mouse) ELISA Kit | 96 Well
Product Overview
Sensitive, Colorimetric Assay
96Trace’s mouse TNF-α ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody (mAb) specific for mouse TNF-α coated on a 96-well plate. Standards and test samples are added to the wells and TNF-α present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TNF-α is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse TNF-α captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml (cell culture supernates), 7.8 pg/ml – 500 pg/ml (mouse serum, plasma). Sensitivity: < 1 pg/ml.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | mouse |
| Sample Type | • Serum & plasma (heparin, EDTA) • Cell culture supernatants |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15.6 pg/ml – 1000 pg/ml (cell culture supernates), 7.8 pg/ml – 500 pg/ml (mouse serum, plasma). Sensitivity: < 1 pg/ml. This ELISA kit shows no cross-reactivity with other relevant proteins |
| Sensitivity | < 1 pg/ml. This ELISA kit shows no cross-reactivity with other relevant proteins |
Applications
Quantitative measurement of TNF-α in mouse serum, plasma, cell lysates, and cell culture supernatants
Features & Benefits
Easy, convenient and time-saving method to assay for mouse TNF-α. Detection Range:15.6 pg/ml – 1000 pg/ml (cell culture supernates), 7.8 pg/ml – 500 pg/ml (mouse serum, plasma). Sensitivity: < 1 pg/ml. This ELISA kit shows no cross-reactivity with other relevant proteins.
Storage & Shipping
| Storage | -20°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | K1051-100 |
| Supplier CAT | TRC-00800 |
| Analyte / Target | TNF-α |
| Category | Cancer Biomarker ELISA Kits |
| Alternate Name | Tumor Necrosis Factor alpha (mouse) ELISA Kit |