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TRC-00671 | Pyruvate Kinase (Human) ELISA Kit | 96 Well

Product Overview

A rapid sandwich ELISA kit for the quantitative measurement of Pyruvate Kinase in human serum, plasma, urine, cell culture samples and other biological fluid

Pyruvate kinase is the enzyme that catalyzes the final step of glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP), yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase is present in four distinct, tissue-specific isozymes, each consisting of particular kinetic properties necessary to accommodate the variations in metabolic requirements of diverse tissues. 96Trace’s PK ELISA kit is a sandwich ELISA assay for the quantitative measurement of PK in human serum, plasma and cell culture supernatants in 90 minutes. The density of color is proportional to the amount of PK captured from the samples.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityHuman
Sample TypeSerum, plasma, urine, cell culture samples, biological fluid.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range8 - 500 pg/ml
Sensitivity< 2.2 pg/ml
Assay Time90 minutes

Applications

This ELISA kit is used for quantitative measurement of total PK in human serum, plasma, urine, cell culture samples, biological fluid.

Features & Benefits

  • Easy, convenient and time-saving method to measure the level of PK in human Serum, plasma, urine, cell culture samples, biological fluid.
  • Detection Range: 8 - 500 pg/ml
  • Sensitivity: < 2.2 pg/ml

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

96Trace CATE4448-100
Supplier CATTRC-00671
Analyte / TargetPyruvate Kinase
CategoryCancer Biomarker ELISA Kits