TRC-00618 | PD-1 (human) ELISA Kit | 96 Well
Product Overview
An ELISA kit for the quantitative measurement of human PD-1 in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids
Human PD-1 (Programmed Death-1) ELISA kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of human PD-1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human PD-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and PD-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PD-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PD-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Applications
This ELISA kit is used for quantitative measurement of PD-1
