IL-33 (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of IL-33 in Plasma,Serum,Tissue lysate,Other biological fluids.

Interleukin-33 (IL-33) belongs to the family of IL-1 cytokines. It is constitutively expressed at increased levels in the nuclei of multiple cell types such as glial, endothelial, smooth muscle, and immune cells. When present in the nucleus, IL-33 functions as a transcriptional repressor, whereas extracellularly, it acts as a traditional cytokine by inducing Th2-type immunity. It also acts as an ‘alarmin’ for the immune system when released by damaged tissues or cell necrosis. Expression of IL-33 is upregulated in cancer and immune-related diseases such as rheumatoid arthritis, asthma, psoriatic arthritis. IL-33 also plays a protective role in cardiovascular and neurodegenerative diseases. Hence, IL-33 could potentially serve as an efficient biomarker for human diseases. 96Trace’s IL-33 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of IL-33 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of IL-33 in the sample or standard.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of IL-33 in Plasma,Serum,Tissue lysate,Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of IL-33.
• Assay format: Sandwich ELISA.
• Sample type: Plasma,Serum,Tissue lysate,Other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 15.625 - 1000 pg/ml,Recovery range: 85 - 105% for normal human serum and plasma samples,Assay Precision: Intra-Assay CV &lt.
• Sensitivity: 9.375 pg/ml,Detection range: 15.625 - 1000 pg/ml,Recovery range: 85 - 105% for normal human serum and plasma samples,Assay Precision: Intra-Assay CV &lt.
• Kit size: 96 Well.

Storage & Shipping

Product Details