TRC-00475 | IL-24 (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for the quantitative measurement of human IL-24 in human serum, plasma and other biological fluids.
IL-24 is induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by IL-24 in melanoma cells, but not in normal immortal melanocytes. 96Trace’s IL-24 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-24. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IL-24 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-24 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-24. The concentration of Human IL-24 in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma,Serum,Other biological fluids |
| Detection Method | Sandwich ELISA, Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15.63-1000 pg/mL,Specificity: No Significant cross-reactivity or interference between Human IL-24 and analogues was observed.,Precision: Coefficient of variation is < |
| Sensitivity | 9.38 pg/mL,Detection Range: 15.63-1000 pg/mL,Specificity: No Significant cross-reactivity or interference between Human IL-24 and analogues was observed.,Precision: Coefficient of variation is < |
Applications
in-vitro quantitative determination of Human IL-24 concentrations in serum, plasma and other biological fluids.
Features & Benefits
Sensitivity: 9.38 pg/mL,Detection Range: 15.63-1000 pg/mL,Specificity: No Significant cross-reactivity or interference between Human IL-24 and analogues was observed.,Precision: Coefficient of variation is < 10%
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4846-100 |
| Supplier CAT | TRC-00475 |
| Analyte / Target | IL-24 |
| Category | Cancer Biomarker ELISA Kits |