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TRC-00361 | Free-PSA (Human) ELISA Kit | 96 Well

Product Overview

A sandwich ELISA kit for the quantitative measurement of Free-PSA in Human serum, plasma, tissue homogenates and other biological fluids.

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme and a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. PSA is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. Most PSA in the blood is bound to serum proteins. A small amount is not protein bound and is called 'free PSA'. Measuring the ratio of free to total PSA appears to be particularly promising for eliminating unnecessary biopsies in men with PSA levels between 4 and 10 ng/mL. 96Trace’s free-PSA ELISA kit is a sandwich ELISA assay for measuring the quantity of human free-PSA in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with fewer incubation and wash step respectively, resulting in less errors and more consistent results.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityHuman
Sample TypeHuman serum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range3 - 200 pg/ml
Sensitivity< 0.5 pg/ml
Assay Time90 minutes

Applications

This ELISA kit is used for quantitative measurement of Free-PSA in Human serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

  • Designed for quantitative measurement of Free-PSA.
  • Assay format: Sandwich ELISA.
  • Sample type: Human serum, plasma, tissue homogenates and other biological fluids.
  • Detection method: Absorbance (450 nm).
  • Detection Range: 3 - 200 pg/ml
  • Sensitivity: < 0.5 pg/ml
  • Assay Time: 90 minutes.
  • Kit size: 96 Well.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

Catalog NumberTRC-00361
Analyte / TargetFree-PSA