FADS2 (Human) ELISA Kit

Product Overview

A Sandwich ELISA kit for quantitative measurement of FADS2 in human serum, plasma, tissue lysates, and other biological fluids

Fatty acid desaturase 2 (FADS2) is a delta-6 fatty acyl CoA desaturase that is involved in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA). This endoplasmic reticulum enzyme is responsible for the conversion of diet-derived linoleic acid and α-linoleic acid into γ-linoleic and stearidonic acid. Subsequently, in the LC-PUFA biosynthesis pathway, it also desaturates tetracosapentaenoate to tetracosahexaenoate, which is then converted to docosahexanoate, an important lipid required for the growth and development of the brain of infants as well as maintaining normal brain function in adults. Studies have demonstrated abnormal expression of FADS2 in cancers such as breast, liver, and lung, leukemia, and melanoma. FADS2 expression correlates with cell proliferation and migration, tumor growth, and angiogenesis. Hence FADS2 could be a promising target for anticancer therapy. 96Trace’s FADS2 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the concentration of FADS2 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of FADS2 in the sample or standard.

Assay Information

Performance Data

Applications

Sandwich ELISA kit to quantitatively measure FADS2 in human serum, plasma, tissue lysate and other biological fluids

Features & Benefits

• Detection range: 78.125 – 5000 pg/ml
• This Sandwich ELISA is highly sensitive and highly specific for the detection of FADS2 in human samples. There is no significant cross-reactivity or interference between FADS2 and analogues
• Recovery range: 85 - 105% for normal human serum and plasma samples
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Sensitivity: 46.875 pg/ml

Storage & Shipping

Product Details