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TRC-00271 | Cytochrome c (Human) ELISA Kit | 96 Well

Product Overview

A sandwich ELISA kit for the quantitative measurement of Cytochrome c in Human serum, plasma, tissue homogenates and other biological fluids.

Cytochrome c is a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the bc1 complex and transfers electrons to the complex IV. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor-1 (Apaf-1). Cytochrome c can also catalyze several redox reactions such as hydroxylation and aromatic oxidation, and shows peroxidase activity by oxidation of various electron donors such as 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 2-keto-4-thiomethyl butyric acid and 4-aminoantipyrine. 96Trace’s Cytochrome c ELISA kit is a sandwich ELISA assay for the quantitative measurement of human Cytochrome c in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human Cytochrome c captured from the samples.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityHuman
Sample TypeHuman serum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range78.12 - 5000 pg/ml
Sensitivity< 46.87 pg/ml

Applications

This ELISA kit is used for quantitative measurement of Cytochrome c in human serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

  • Designed for quantitative measurement of Cytochrome c.
  • Assay format: Sandwich ELISA.
  • Sample type: Human serum, plasma, tissue homogenates and other biological fluids.
  • Detection method: Absorbance (450 nm).
  • Detection Range: 78.12 - 5000 pg/ml
  • Sensitivity: < 46.87 pg/ml
  • Kit size: 96 Well.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

Catalog NumberTRC-00271
Analyte / TargetCytochrome c